ELISA
- Created by: I Can't Revise!
- Created on: 10-05-18 19:31
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- ELISA
- Step 1:
- Assay plate is coated with a known antigen.
- Step 2:
- Patient's serum sample is used to fill the wells.
- Step 5:
- Enzyme-linked antibody solution is then added to the wells. Enzyme-linked antibodies bind to human antibodies.
- Step 3:
- Specific antibody for known antigen binds to antigen.
- Step 4:
- Non-specific (unbound) antigen is washed away.
- Step 6:
- Wells washed to remove unbound enzyme-linked antibodies.
- Step 7:
- Substrate specific for the enzyme is added and binds. Binding causes colour change.
- Stands for: Enzyme-linked immunosorbent assay
- Is used to diagnose: HIV, Herpes, Viruses
- Step 1:
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