ELISA

HideShow resource information
View mindmap
  • ELISA
    • Step 1:
      • Assay plate is coated with a known antigen.
    • Step 2:
      • Patient's serum sample is used to fill the wells.
    • Step 5:
      • Enzyme-linked antibody solution is then added to the wells. Enzyme-linked antibodies bind to human antibodies.
    • Step 3:
      • Specific antibody for known antigen binds to antigen.
    • Step 4:
      • Non-specific (unbound) antigen is washed away.
    • Step 6:
      • Wells washed to remove unbound enzyme-linked antibodies.
    • Step 7:
      • Substrate specific for the enzyme is added and binds. Binding causes colour change.
    • Stands for: Enzyme-linked immunosorbent assay
    • Is used to diagnose: HIV, Herpes, Viruses

Comments

No comments have yet been made

Similar Applied Science resources:

See all Applied Science resources »See all ELISA test resources »