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  • Created by: rebecccab
  • Created on: 17-05-16 08:28

HOW ELISA TESTS ARE CARRIED OUT

ELISA tests are carried out in special plates that have an ordered arrangement of wells on their surface

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HOW ELISA TESTS ARE CARRIED OUT

The following outlines one way in which an ELISA test can be used to find a pathogen in a
person’s blood serum

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HOW ELISA TESTS ARE CARRIED OUT

Each well of the plate is coated with the antigen of the specific disease which is being
looked for

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HOW ELISA TESTS ARE CARRIED OUT

The well is then washed to remove surplus antigen

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HOW ELISA TESTS ARE CARRIED OUT

The patient’s serum is added to the well

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HOW ELISA TESTS ARE CARRIED OUT

Any antibodies specific to the disease will bind to the antigens at the bottom of the
well

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HOW ELISA TESTS ARE CARRIED OUT

The well is then washed again to remove any antibodies that have not attached
themselves to antigens

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HOW ELISA TESTS ARE CARRIED OUT

An antibody complex is then added.  This can be a fluorescent marker or a
radioactive marker; in the case of ELISA, an enzyme is added

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HOW ELISA TESTS ARE CARRIED OUT

The antibody complex (enzyme) binds to the antibody

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HOW ELISA TESTS ARE CARRIED OUT

The well is washed again to remove any unbound enzymes

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HOW ELISA TESTS ARE CARRIED OUT

Finally a substrate is added and is converted by the enzyme into a coloured product

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HOW ELISA TESTS ARE CARRIED OUT

The colour change indicates the presence of the pathogen

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