- effects of temp, substrate concentration and pH on rate of reaction
- rate of reaction=amount of substrate used or amount of product made÷time taken
- at lower temps molecules move slower.
- at the optimum temp the enzyme is working at its fastest rate.
- higher temps cause active site to change shape so reaction goes slower
- at very high temps the active site breaks up and the enzyme denatures
- substrate concentration
- beginning of reaction not every active site enzyme is busy. adding more substrate increases rate of reactions.
- Changes in pH alters the shape of enzyme’s active site.
- Each enzyme work bests at a specific pH value.
- optimum pH of enzyme depends on where it works. enzyme in the small intestine have optimum pH of 7.5
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