effects of temperature

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  • effects of temp, substrate concentration and pH on rate of reaction
    • rate of reaction=amount of substrate used or amount of product made÷time taken
    • temp
      • at lower temps molecules move slower.
      • at the optimum temp the enzyme is working at its fastest rate.
      • higher temps cause  active site to change shape so reaction goes slower
      • at very high temps the active site breaks up and the enzyme denatures
    • substrate concentration
      • beginning of reaction not every active site enzyme is busy. adding more substrate increases rate of reactions.
    • pH
      • Changes in pH alters the shape of enzyme’s active site.
      • Each enzyme work bests at a specific pH value.
      • optimum pH of enzyme depends on where it works. enzyme in the small intestine have optimum pH of 7.5


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