visualising cells; microscopy and staining

  • Created by: molg
  • Created on: 16-05-18 16:43
spatial resolution
ability to distinguish between 2 small objects
1 of 11
Abbe's law of limiting resolutions
wavelength of light / numerical aperature
2 of 11
numerical aperature
ability to collect light ( refractive index of medium X angular width of cone of light entering objective lens)
3 of 11
Fluorescent microscope
energy of photon is inversely propotional to wavelength
4 of 11
GFP
absorbs blue light and releases green light
5 of 11
stokes shift
shorter wavelength in- longer wavelength out
6 of 11
chemical dyes
eg fluo-4= calcium indicator
7 of 11
diolistics
delivery (of dye) on high speed gold particles
8 of 11
Genetically- encoded proteins (GFP)
transfection by chemical/electrical means, biolistics (on high speed gold particles), viral infection, transgenic animals
9 of 11
Wildfield microscopy
the whole specimen on the microscope is exposed to a light
10 of 11
Confocal microscopy
Point-by-point by scanning + pinhole rejects out-of-focus light
11 of 11

Other cards in this set

Card 2

Front

wavelength of light / numerical aperature

Back

Abbe's law of limiting resolutions

Card 3

Front

ability to collect light ( refractive index of medium X angular width of cone of light entering objective lens)

Back

Preview of the back of card 3

Card 4

Front

energy of photon is inversely propotional to wavelength

Back

Preview of the back of card 4

Card 5

Front

absorbs blue light and releases green light

Back

Preview of the back of card 5
View more cards

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