Magnification = size of image / actual size of object
1 of 15
Definition of resolution
Ability to distinguish between two separate points close together
2 of 15
What is the difference in wavelength between an optical microscope and an electron microscope
An electron microscope has a much shorter wavelength
3 of 15
How does a shorter wavelength effect the use of the microscope
It increases the resolution and magnification
4 of 15
Why must specimens in an electron microscope be dried out?
Because water boils at room temperature in the vacuum
5 of 15
Why is the solution kept cold in cell fractionation?
To reduce enzyme activity
6 of 15
Why is a buffer added to the solution
To maintain a constant pH
7 of 15
Why is the solution added isotonic?
To prevent organelles bursting or shrinking by osmosis
8 of 15
Name of the equipment which separates the organelles in ultracentrifugation by spinning solution at different speeds
Ultracentrifuge
9 of 15
Name of the fluid removed after each spin containing low density organelles
Supernatant
10 of 15
Out of light or electron microscopes which have a higher resolution?
Electron Microscope
11 of 15
Out of light or electron microscope which have a greater magnification?
Electron Microscope
12 of 15
Which electron microscope uses electromagnets to focus a beam of electrons through the specimen which shows different densities as denser substances absorb more electrons?
Transmission Electron Microscope (TEM)
13 of 15
Which electron microscope scan a beam of electrons across the surface of the specimen to show the 3D surface of a specimen
Scanning Electron Microscope (SEM)
14 of 15
Out of SEM or TEM which gives a higher resolution?
TEM
15 of 15
Other cards in this set
Card 2
Front
Definition of resolution
Back
Ability to distinguish between two separate points close together
Card 3
Front
What is the difference in wavelength between an optical microscope and an electron microscope
Back
Card 4
Front
How does a shorter wavelength effect the use of the microscope
Back
Card 5
Front
Why must specimens in an electron microscope be dried out?
Comments
No comments have yet been made