Studying Cells - Microscopes

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Formula for magnification
Magnification = size of image / actual size of object
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Definition of resolution
Ability to distinguish between two separate points close together
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What is the difference in wavelength between an optical microscope and an electron microscope
An electron microscope has a much shorter wavelength
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How does a shorter wavelength effect the use of the microscope
It increases the resolution and magnification
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Why must specimens in an electron microscope be dried out?
Because water boils at room temperature in the vacuum
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Why is the solution kept cold in cell fractionation?
To reduce enzyme activity
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Why is a buffer added to the solution
To maintain a constant pH
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Why is the solution added isotonic?
To prevent organelles bursting or shrinking by osmosis
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Name of the equipment which separates the organelles in ultracentrifugation by spinning solution at different speeds
Ultracentrifuge
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Name of the fluid removed after each spin containing low density organelles
Supernatant
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Out of light or electron microscopes which have a higher resolution?
Electron Microscope
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Out of light or electron microscope which have a greater magnification?
Electron Microscope
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Which electron microscope uses electromagnets to focus a beam of electrons through the specimen which shows different densities as denser substances absorb more electrons?
Transmission Electron Microscope (TEM)
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Which electron microscope scan a beam of electrons across the surface of the specimen to show the 3D surface of a specimen
Scanning Electron Microscope (SEM)
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Out of SEM or TEM which gives a higher resolution?
TEM
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Other cards in this set

Card 2

Front

Definition of resolution

Back

Ability to distinguish between two separate points close together

Card 3

Front

What is the difference in wavelength between an optical microscope and an electron microscope

Back

Preview of the front of card 3

Card 4

Front

How does a shorter wavelength effect the use of the microscope

Back

Preview of the front of card 4

Card 5

Front

Why must specimens in an electron microscope be dried out?

Back

Preview of the front of card 5
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