Investigation of Cell Structure

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Magnification=
Length of image / Length of Specimen
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Smallest unit of measure
Nanometre
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Magnification is...
how much bigger an image is than the specimen
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Resolution
how a microscope distinguishes between two points that are close together
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Length of image
Magnification * Length of Specimen
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Homogenisation
Breaking up the cells
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Filteration
Separating cell debris or tissue debris, like connective tissue, from the organelles.
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Ultracentrifugation
Separating Organelles
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During Homogenization the solution is kept ice cold
to reduce enzyme activity that break down organelles
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During Homogenization the solution is kept isotonic
to prevent the movement of water in and out of organelles by osmosis
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Isotopic means
same concentration of chemicals as the cells being broken down
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During Homogenization a buffer is added to the solution
to prevent changes in pH
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Order of which organelles are separated during ultracentrifugation
Nuclei, Mitohondria, Lysosome, ER, Ribosomes
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1000µm
1mm
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Which has the shortest wavelength? (Light or Electrons)
Electrons
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TEMs stands for
Transmission electron microscope
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SEMs stands for
Scanning electron microscope
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Advantage of SEMs
Can be used on thick specimen.
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Advantage of SEMs
Can produce 3D images
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Disadvantage of both SEMs and TEMs
Can only be used on non-living things
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Other cards in this set

Card 2

Front

Smallest unit of measure

Back

Nanometre

Card 3

Front

Magnification is...

Back

Preview of the front of card 3

Card 4

Front

Resolution

Back

Preview of the front of card 4

Card 5

Front

Length of image

Back

Preview of the front of card 5
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