Genetics - Cloning

?
  • Created by: mivawy
  • Created on: 04-11-21 08:09
example of proteins made from cloned genes
growth hormone
1 of 35
example of proteins made from cloned genes
insulin
2 of 35
advantage of producing proteins from clones genes
consistency
3 of 35
cloning in nature
asexual reproduction
4 of 35
cloning in nature
identical twins
5 of 35
Clone
exact genetic copies
6 of 35
Defined as the deliberate production of genetically identical individuals
Reproductive cloning
7 of 35
Creates a viable embryo from a body cell and an egg cell
Somatic Nuclear Transfer
8 of 35
Cell is implanted into the uterus of a surrogate female parent where embryotic development continues
Embryo Transfer
9 of 35
Problems with reproductive cloning
Premature aging
10 of 35
Purpose is to produce stem cells for use in treatment of injury or disease
Therapeutic Cloning
11 of 35
undifferentiated cells
stem cells
12 of 35
used for Type 1 diabetes or parkinson's
stem cells
13 of 35
Genetic information is changed by inserting a new gene
Genetic modification
14 of 35
negative charge
Phosphates
15 of 35
A pairs with
T
16 of 35
G pairs with
C
17 of 35
Strong covalent bonds hold the phosphates and the sugars together
Phosphodiester bond
18 of 35
universal code
genetic code
19 of 35
cut at a specific sequence of DNA
restriction enzyme
20 of 35
The restriction enzymes cut at a specific sequence of DNA called
recognition site
21 of 35
straight cut
blunt
22 of 35
staggered cut
sticky
23 of 35
joins DNA or RNA strands together
ligase
24 of 35
The first step in PCR
denature
25 of 35
The last step in PCR
Elongation
26 of 35
The 2nd step in PCR
Annealing
27 of 35
temperature and duration of the first PCR stage (number duration[mins/hours/seconds])
94 2min
28 of 35
55 °C for two minutes
Annealing
29 of 35
A technique that separates DNA fragments based on their molecular size
Gel Electrophoresis
30 of 35
1 band represent
homozygous allele
31 of 35
2 bands represent
heterozygous allele
32 of 35
advantage of producing proteins from cloned genes
purity
33 of 35
gene of interest, plasmid, cut, ligase
transforming DNA
34 of 35
Isolate mRNA from cell - this is for _______
copying DNA
35 of 35

Other cards in this set

Card 2

Front

example of proteins made from cloned genes

Back

insulin

Card 3

Front

advantage of producing proteins from clones genes

Back

Preview of the front of card 3

Card 4

Front

cloning in nature

Back

Preview of the front of card 4

Card 5

Front

cloning in nature

Back

Preview of the front of card 5
View more cards

Comments

No comments have yet been made

Similar Biology resources:

See all Biology resources »See all Biology resources »