FM - Monitoring Bacteria - Flashcards in University Biology

1. What does acetone do in gram staining?
Dissolves the lipids that make up the outer membrane of gram (1) bacteria. It dehydrates the peptidoglycan layer found in (2). The Crystal Violet- Iodine complexes cannot be retained so the dye leaks out of gram (3)

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These are taken up by negative cell types where the Cl^- ions bind and stain the negatively charged components of the bacterial cell wall. Only gram -ve cells are purple.
These are taken up by both cell types where the CV ^+ ions bind and stain the negatively charged components of the bacterial cell wall. Both gram +ve and -ve cells are purple.
These are taken up by positive cell types where the CV^+ ions bind and stain the negatively charged components of the bacterial cell wall. Only gram +ve cells are purple.

Taken up by both cell types, binds to lipid cell membrane and counterstains. Gram negative go from colourless to pink
Taken up by negative cell types, binds to lipid cell membranes and counterstains, gram negatives go from pink to colourless.
Taken up by negative cell types, binds to lipid cell membrane and counterstains. Gram negative go from colourless to pink.
Taken up by both cell types, binds to lipid cell membranes and counterstains, gram negative go from pink to colourless.