6. Electrophoresis relies on the fact that DNA is...
Negatively Charged
Positively Charged
Has no charge
7. Which of these is NOT a vector into which fragments of DNA are normally incorporated?
Virus genomes
Plant cell
Yeast cell chromosomes
E.coli
8. What enzyme is is NOT used in the Engineering of Human Insulin?
RNA polymerase
DNA polymerase
DNA ligase
Reverse Transcriptase
9. The cycle of temperatures used in PCR is...
92, 75, 55
95, 55, 72
95, 72, 55
72, 55, 95
55, 75, 95
10. The enzyme DNA polymerase used in PCR can be described as...
Thermophobic
Hydrophilic
Thermophilic
Hydrophobic
11. DNA grows from the 5' end
False
True
12. The use of lasers features in...
Genetic Engineering
Electrophoresis
Automated DNA Sequencing
PCR
13. Which is the true statement?
DNA ligase seals the phosphate-sugar backbone
DNA ligase is not the same enzyme that is used in natural DNA replication
DNA ligase anneals sticky ends
DNA ligase is used in PCR
14. To encourage bacterial cells to become transformed and transgenic we use heat shock and....
Calcium Salts
Phosphates
Potassium Salts
Magnesium Salts
15. To genetically engineer insulin scientists find the...
mRNA for the gene
DNA for the gene
cDNA for the gene
16. Which is a true statement about the transformed/transgenic bacteria (those that have taken up a plasmid with the insulin gene) used in genetically engineering insulin?
They will grow on amypicillin but not tetracycline agar
They will grow on both ampicillin and tetracycline agar
They will grow on tetracycline but not ampicillin agar
They will not grow on tetracycline or ampicillin agar
17. To cure the symptoms of Cystic Fibrosis we would use...
