cell structure methods of studying cells 3.1/3.2

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what is magnification
The magnification is how many times bigger the image is when compared with the object
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what is the equation for magnification
size of image/ size of object
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What is the resolution
Is the minimum distance apart that two objects can be in order to appear as separate items
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what is cell fractionation
Its the process where cells are broken up and the different organelles they contain are separated out
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In cell fractionation why are cells placed in a cold solution
to reduce enzyme activity
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why is the solution the same water potential as the tissues
To prevent organelles bursting or shrinking as a result of osmosis
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why is the solution buffered
So the ph does not fluctuate and alter the structure of organelles
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what is homogenation
Cells are broken down by a homogeniser and it gives out organelles the fluid is called is a homogenate
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what is ultracentrifugation
This is where fragments are separated in a machine called a centrifuge
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what are the steps of ultracentrifugation
The tube filtrate is placed in a centrifuge and spun at low speeds,heavy organelles forced to bottom, fluid removed and transferred to another tube
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what is this fluid called in ultracentrifugation
the supernatant
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true or false electron microscopes have a high resolving power
true
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true or false light microscopes have a good resolution
false
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true or false the electron microscope uses light to form an image
false
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what are the two types of electron microscopes
Transmission and scanning
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true or false for an a TEM a thin specimen should be used
true
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what happens high energy electrons are used on the specimen
it could get destroyed
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what are the main limitations of a TEM
must be in a vacuum,a staining process is required, specimen must be thin,may contain artefacts
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what are artefacts
Are things that result from the way the specimen is prepared
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True or false in an SEM a thin specimen is also used
false
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true or false the SEM has a lower resolving power than the TEM
true
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what is the equation for magnification

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size of image/ size of object

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What is the resolution

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what is cell fractionation

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In cell fractionation why are cells placed in a cold solution

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