Methionine due to sulfur. it is oxidised to methionin sulfoxide which regulate protein function. It can be switched of by oxidising again to sulfone O=S=O this reaction is non reversible and requires msrA/B and NADPH to reduce.
1 of 12
How is methionine first oxidised?
Due to a OH radical from H2O2. this reaction is reversible
2 of 12
How are hydrophilic alcohols regulated?
Serine, tyrosine and threonine are phosphorylated to be regulated. GFP serine, tyrosine and glycine attack in the presence of oxygen and fluoresce
3 of 12
How does cysteine react in oxidation?
Like an alcohol but instead forming R-S-S-R bonds.
4 of 12
What is farnesylation?
When farnesyltransferase attaches to isoprene units that can attch to sulfur. In RAS genes.
5 of 12
What us glycosylation?
sugar attaches to N (asparagine) or O (serine). occurs in blood antigens.
6 of 12
Mechanism of glycosylation?
1. Activation reaction. sugar group attach to UDP to lift energy and drive equilibrium to product. 2. Reaction with protein. sugar transferred from UDP to oxygen on serine. Nucleophillic attack of serine. Phosphate group takes H from OH on serine
7 of 12
continued.
and the sugar group can attach.
8 of 12
What is pKa for acids and bases
below 7 for acids and above 7 for bases.
9 of 12
What happens during protein synthesis?
Nucleophilic attack of amino group from new aa on carboxyl group of other aa. Peptidyltransferase holds the aa in place.
10 of 12
What is antibiotic pyromycin
It resembles the shape of tRNA and can prevent protein synthesis,
11 of 12
What is the Maillard reaction?
When sugars and proteins combine when heated to form amino ketose which forms nice flavours and smells when heating meat, bread etc.
12 of 12
Other cards in this set
Card 2
Front
How is methionine first oxidised?
Back
Due to a OH radical from H2O2. this reaction is reversible
Comments
No comments have yet been made