Bacteria and Archaea 0.0 / 5 ? OtherMicrobiologyUniversityNone Created by: MariaC5Created on: 03-01-18 19:43 Which is not a microscopic observation? Age 1 of 38 What is the most widely used differential staining procedure? Gram stain 2 of 38 Which test divides bacteria into two groups (G+ & G-) based on differences in cell wall structure? Gram stain 3 of 38 In an oxidase test, positive results show as what colour? Purple 4 of 38 In an oxidase test, negative results show as what colour? Colourless 5 of 38 What does the oxidation/fermentation test look at? Glucouse 6 of 38 What is the medium in culture media? Nutrient solution for growing microorganisms 7 of 38 What is the inoculum in culture media? Initial small number of cells added grown to give a culture 8 of 38 What is agar in culture media? Polysaccharide extracted from seaweed 9 of 38 At what temperature does agar melt? 100'C 10 of 38 Agar remains molten down to... 50'C 11 of 38 What are the two categories of media? Complex and synthetic/defined 12 of 38 What does the category of media, complex mean? Undefined reagents 13 of 38 A complex selective media means? it contains an agent that only allows growth of certain organisms 14 of 38 A complex differential media means? the agent has changed in some visible way 15 of 38 What does synthetic/defined mean? All chemical components known 16 of 38 What allows the growth of particular microorganisms while inhibiting the growth of others? Selective agents 17 of 38 Bile salts are an example of Selective agents 18 of 38 Crystal violet is an example of Selective agents 19 of 38 NaCl is an example of Selective agents 20 of 38 Chloramphenical is an example of Selective agents 21 of 38 Blood is an example of Differential agents 22 of 38 Fermentable carbohydrate plus pH indicator is an example of what? Differential agents 23 of 38 What is the approximate diameter for a red blood cell? 7000nm 24 of 38 What is the approximate WxL of E.coli 1300nm x 4000nm 25 of 38 What is the approximate diameter of streptococcus? 800nm-1000nm 26 of 38 In a bacterial cell, the cell envelope has how many layers? 3 27 of 38 What is not a layer in the cell envelope of a bacterial cell? Fluid layer 28 of 38 What is not another common features of a bacterial cell? Internal structures 29 of 38 Peptidoglycan is a unique compound of what? Bacterial cell wall 30 of 38 What is peptidoglycan? A rigid structure that lies just outside the cell plasma membrane 31 of 38 If the gram stain is positive what can be assumed about the peptidoglycan? It is thick 32 of 38 If the gram stain is negative what can be assumed about the peptidoglycan and outer membrane? It is thin 33 of 38 In the bacterial cell wall what are the two alternating amino acids? D- and L- 34 of 38 Gram positive cell walls are composed mainly of? Peptidoglycan 35 of 38 Gram positive cell walls also contain? Teichoic acids 36 of 38 What does Lysozyme in the cell wall do? Breaks the bond between N-acetyl glucosamine and N-actylmuramic acid 37 of 38 What does penicillin do? Inhibits peptidolgycan synthesis 38 of 38
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