Back to quiz

6. Which of these measurements is not presented on a logarithmic scale?

  • Immunofluorescence
  • Viability dye measurements
  • Cell cycle analysis
  • Comparisons of two different cell populations

7. Which of these organs cannot be used to produce a white cell suspension?

  • Lymph node
  • Thymus
  • Kidney
  • Spleen

8. Which lymph nodes are located under the arms?

  • Cervical lymph nodes
  • Brachial lymph nodes
  • Inguinal lymph nodes
  • Popliteal lymph nodes

9. Which is not an example of a vital dye?

  • Haematoxylin and eosin
  • 7-AAD
  • Trypan blue
  • Propium iodide

10. Which of these is not a function of the electronics system on a flow cytometry machine?

  • Converts reflected light into electrons
  • Interprets results
  • Performs compensation
  • Converts analog systems to digital

11. Which is a type of detector found within a flow cytometry machine?

  • Proximity
  • Photovoltaic
  • Photodiode
  • Light dependent

12. What does forward scatter in flow cytometry show?

  • All of the above
  • Cell size
  • A chosen marker
  • Cell complexity

13. Which of these is not a key feature of apoptosis?

  • Organelle leakage
  • Mitochondrial depolarisation
  • Membrane blebbing
  • Nuclear fragmentation

14. Which marker is used to label astrocytes?

  • Glial fibrillary acidic protein
  • Ionised calcium binding adaptor molecule
  • Neurofilament
  • Myelin basic protein

15. Which particle characteristics are measured by flow cytometry?

  • All of the above
  • Chemical
  • Physical
  • Functional

16. Which event in apoptosis is measured using Annexin-V?

  • Nuclear fragmentation
  • Apoptosome formation
  • Phosphatidyl-serine translocation
  • Membrane blebbing

17. What is doublet discrimination associated with?

  • Dead cells
  • Poor sample preparation
  • Computer errors
  • Fluidics errors

18. Which is the best way to determine positive cells in an experiment?

  • Gating
  • Fluorescence minus one
  • Compensation beads
  • Isotype controls

19. What is the role of caspase 1 in pyroptosis?

  • Activates complement cascades
  • Creates pores in cell membrane for cytokine release
  • Downregulates MHC I to encourage NK cell killing
  • Creates Fc receptors to promote opsonisation

20. Which parameter is the threshold usually set on in flow cytometry?

  • FSC-A
  • SSC-A
  • FSC-W
  • SSC-W