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6. Which of these measurements is not presented on a logarithmic scale?
- Immunofluorescence
- Viability dye measurements
- Cell cycle analysis
- Comparisons of two different cell populations
7. Which of these organs cannot be used to produce a white cell suspension?
- Lymph node
- Thymus
- Kidney
- Spleen
8. Which lymph nodes are located under the arms?
- Cervical lymph nodes
- Brachial lymph nodes
- Inguinal lymph nodes
- Popliteal lymph nodes
9. Which is not an example of a vital dye?
- Haematoxylin and eosin
- 7-AAD
- Trypan blue
- Propium iodide
10. Which of these is not a function of the electronics system on a flow cytometry machine?
- Converts reflected light into electrons
- Interprets results
- Performs compensation
- Converts analog systems to digital
11. Which is a type of detector found within a flow cytometry machine?
- Proximity
- Photovoltaic
- Photodiode
- Light dependent
12. What does forward scatter in flow cytometry show?
- All of the above
- Cell size
- A chosen marker
- Cell complexity
13. Which of these is not a key feature of apoptosis?
- Organelle leakage
- Mitochondrial depolarisation
- Membrane blebbing
- Nuclear fragmentation
14. Which marker is used to label astrocytes?
- Glial fibrillary acidic protein
- Ionised calcium binding adaptor molecule
- Neurofilament
- Myelin basic protein
15. Which particle characteristics are measured by flow cytometry?
- All of the above
- Chemical
- Physical
- Functional
16. Which event in apoptosis is measured using Annexin-V?
- Nuclear fragmentation
- Apoptosome formation
- Phosphatidyl-serine translocation
- Membrane blebbing
17. What is doublet discrimination associated with?
- Dead cells
- Poor sample preparation
- Computer errors
- Fluidics errors
18. Which is the best way to determine positive cells in an experiment?
- Gating
- Fluorescence minus one
- Compensation beads
- Isotype controls
19. What is the role of caspase 1 in pyroptosis?
- Activates complement cascades
- Creates pores in cell membrane for cytokine release
- Downregulates MHC I to encourage NK cell killing
- Creates Fc receptors to promote opsonisation
20. Which parameter is the threshold usually set on in flow cytometry?