- Before cell fractionation actually takes place, the tissue is placed in a isotonic buffer:
COLD- to reduce enzyme activity.
ISOTONIC- to prevent organelles from bursting or shrinking; it should have the same water potential as the tissue sample- to prevent water moving in or out of cells.
BUFFER- to maintain the pH- prevent enzymes from denaturing- changing structure.
- Cell broken up by homogeniser to release the organelles from the cells of…