Cells and Transport

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CELL FRACTIONATION

  • Before cell fractionation actually takes place, the tissue is placed in a isotonic buffer:

COLD- to reduce enzyme activity.

ISOTONIC- to prevent organelles from bursting or shrinking; it should have the same water potential as the tissue sample- to prevent water moving in or out of cells.

BUFFER- to maintain the pH- prevent enzymes from denaturing- changing structure.

HOMOGENATION

  • Cell broken up by homogeniser to release the organelles from the cells of

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