Microscopes

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Microscopy

  • Eyepiece - magnification lens
  • Carosel - different magnifications - objective lens
  • Overall Magnification = Eyepiece magnification X objective magnification
  • Magnification- The process of enlarging the appearance of somethng without nessacarily revealing more detail
  • Resolution- In microscopy the minimum distance that two points can be sperated and still be distinguished as two seperate points
  • Image size= Actual Size X Magnification
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Light Microscopes

  • Maximum magnification of X1500 (usually)
  • Maximum resolution is 0.2 um

Advantages

  • Relatively Inexpensive
  • View varity of samples
  • Shows Colour

Disadvantages

  • Relatively low magnification
  • Resoloution is lower for viewing smaller things
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Transmission Electron Microscopes (TEM)

  • 2D image
  • maximum magnification X500,000
  • electron beam passes through a small sample and is detected

Advantages

  • Much higher resolution than light microscopes
  • Much higher magnification than light microscopes - higher detail

Disadvantages

  • Small objects have to be placed in a vacum
  • very expensive to buy and maintain
  • have to have skilled personel in order to prepare it
  • Only black and white image


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Scanning Electron Microscopes

  • 3D image - multiple detectors
  • maximum magnification - X100,000
  • electrons bounce off  speciemens

Advantages

  • Much higher resolution than light microscopes
  • Much higher magnification than light microscopes - higher detail
  • 3D image

Disadvantages

  • Small objects have to be placed in a vacum
  • very expensive to buy and maintain
  • have to have skilled personel in order to prepare it
  • Only black and white image
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Electron Microscopes

Electron microscopes have a lower wavelegnth than light microscopes therefore can produce a greater resoloution up to 0.1nm

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