Microscopes 0.0 / 5 ? BiologyCellular processes and structureASAQA Created by: bethadams86Created on: 27-11-13 16:08 Microscopy Eyepiece - magnification lens Carosel - different magnifications - objective lens Overall Magnification = Eyepiece magnification X objective magnification Magnification- The process of enlarging the appearance of somethng without nessacarily revealing more detail Resolution- In microscopy the minimum distance that two points can be sperated and still be distinguished as two seperate points Image size= Actual Size X Magnification 1 of 5 Light Microscopes Maximum magnification of X1500 (usually) Maximum resolution is 0.2 um Advantages Relatively Inexpensive View varity of samples Shows Colour Disadvantages Relatively low magnification Resoloution is lower for viewing smaller things 2 of 5 Transmission Electron Microscopes (TEM) 2D image maximum magnification X500,000 electron beam passes through a small sample and is detected Advantages Much higher resolution than light microscopes Much higher magnification than light microscopes - higher detail Disadvantages Small objects have to be placed in a vacum very expensive to buy and maintain have to have skilled personel in order to prepare it Only black and white image 3 of 5 Scanning Electron Microscopes 3D image - multiple detectors maximum magnification - X100,000 electrons bounce off speciemens Advantages Much higher resolution than light microscopes Much higher magnification than light microscopes - higher detail 3D image Disadvantages Small objects have to be placed in a vacum very expensive to buy and maintain have to have skilled personel in order to prepare it Only black and white image 4 of 5 Electron Microscopes Electron microscopes have a lower wavelegnth than light microscopes therefore can produce a greater resoloution up to 0.1nm 5 of 5
Comments
Report