1) To take extracts from the desired plant: dry and grind each plant, and then soak them in ethanol. (ethanol acts as a solvent). The plants should all be the same size, so the amount of extract is the same.
2) Filter off the liquid (the ethanol containing the dissolved plant extract).
3) Evenly spread a sample of bacteria onto an agar plate.
4) Dip discs of absorbant paper in each extract. The discs should all be the same size so that they absorb the same volume of liquid.
5) Also make a control disc, which has been soaked in ethanol.
6) Place the paper discs on the agar plate- they must be spread out.
7) Incubate the bacteria to allow the bacteria to grow.
8) Where the bacteria can't grow- a clear patch will form called an incubation zone.
9) The size of the incubation zone tells you how well the antimicrobial plant is extract is working.
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