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Learning Objectives
· Recap how DNA probes and DNA hybridisation
is used to locate specific genes.
· Learn how the exact order of nucleotides on a
strand of DNA can be determined.
· Learn how restriction mapping can be used to
determine nucleotide sequences.…read more

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DNA Probes
· DNA probes are simple, short and single-stranded sections of
DNA.
· They will bind to complementary sections of other DNA
strands.
· Due to being labelled in some way, they make this `other
DNA' easily identifiable.
Labelling with radioactivity Labelling with fluorescence…read more

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Remember that probes can be used as an
easy method of screening (detecting) for
mutated genes.
But also remember that the probe needs to
be complementary to the mutated gene.
So this means, that to produce a probe, you
first need to sequence your gene.
How do we sequence genes?…read more

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Meet Frederick Sanger...
· Biochemist
· Cambridge University
· English
· Two Nobel Prizes
· Still Alive
Sanger's work in the 1970's, which
earned him his second Nobel Prize,
involved the sequencing of DNA.
His method used modified nucleotides that do now allow
another nucleotide to join after them in a sequence.
Sanger Sequencing Method…read more

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Introducing Sanger Sequencing
· The method is based on the premature ending of DNA
synthesis.
· If modified nucleotides are used during DNA synthesis, the
process can be halted.
What normally happens during DNA synthesis...
T A T G G A T C T G A C C T T A G
A T A C C T A G A C T G G A A T C
What happens if you modify a nucleotide...
You call these modified
T A T G G A T C nucleotides, TERMINATORS
A T A C C T A G A C T G G A A T C…read more

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Comments

Jess Doran

This is a great powerpoint and has helped me understand locating and sequencing so much better! Thank you so much! **

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