Affect of temperature on membrane permiability
- Created by: Emling
- Created on: 22-03-19 09:12
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- Effect of temperature on membrane permeability- Core Practical
- Method
- 1. Use a scalpel to cut 5 equal size pieces of beetroot
- 2. Rinse the beetroot to remove any pigment released during cutting
- 3. Use a measuring cylinder to measure 5 cm cubed of water into 5 test tubes
- 4. Place beet root into the test tubes and place the test tubes into 5 different water baths (10,20,30,40,50)
- 5. Remove the beetroot and place the coloured liquid in a colorimetre
- Equiptment
- Scaple
- Beetroot
- water
- Measuring cylinder
- Test tubes
- Water baths
- Colourimetre
- Variables
- Independent
- Temperature of the water baths
- Dependent
- How much colour (betalain) leaks out of a beetroot
- Control
- Time that the beetroot is in the water
- Will affect how much the membranes denature
- Size of beetroot
- So results from different temperatures can be compared
- Volume of liquid beetroot is put in
- Will affect how dilute the coloured liquid is
- Type of liquid beetroot is put into
- Can affect how much the membrane denatures
- Type of beetroot
- All have slightly different membranes
- Time that the beetroot is in the water
- Independent
- Method
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