visualising cells; microscopy and staining 0.0 / 5 ? Biologyvisualising cells; microscopy and stainingUniversityNone Created by: molgCreated on: 16-05-18 16:43 spatial resolution ability to distinguish between 2 small objects 1 of 11 Abbe's law of limiting resolutions wavelength of light / numerical aperature 2 of 11 numerical aperature ability to collect light ( refractive index of medium X angular width of cone of light entering objective lens) 3 of 11 Fluorescent microscope energy of photon is inversely propotional to wavelength 4 of 11 GFP absorbs blue light and releases green light 5 of 11 stokes shift shorter wavelength in- longer wavelength out 6 of 11 chemical dyes eg fluo-4= calcium indicator 7 of 11 diolistics delivery (of dye) on high speed gold particles 8 of 11 Genetically- encoded proteins (GFP) transfection by chemical/electrical means, biolistics (on high speed gold particles), viral infection, transgenic animals 9 of 11 Wildfield microscopy the whole specimen on the microscope is exposed to a light 10 of 11 Confocal microscopy Point-by-point by scanning + pinhole rejects out-of-focus light 11 of 11
Principles of diagnostic tests and associated specimens. Clinical reports and sending isolates away for further identification. 0.0 / 5
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