Using Restriction Endonucleases.

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What are restriction endonucleases?
When a bacteria is attacked by a virus which injects it's DNA, some bacteria produce an enzyme that cuts up the viral DNA.This is a restriction endonuclease.
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Why are there many different types of restriction endonucleases?
Because each one cuts a double DNA strand at a specific sequence.
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What are 'blunt edges'?
When restriction endonucleases cut between two opposite base pairs leaving two straight edges called blunt ends.
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What are 'sticky ends'?
When restriction endonucleases cut DNA in a staggered fashion leaving exposed unpaired bases.
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What is a palendromic sequence?
A sequence of 6 nucleotides that is the same read left and read right.
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What is the recognition site of a DNA sequence called if it is palendromic?
It is a 6bp palindromic sequence.
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Other cards in this set

Card 2

Front

Why are there many different types of restriction endonucleases?

Back

Because each one cuts a double DNA strand at a specific sequence.

Card 3

Front

What are 'blunt edges'?

Back

Preview of the front of card 3

Card 4

Front

What are 'sticky ends'?

Back

Preview of the front of card 4

Card 5

Front

What is a palendromic sequence?

Back

Preview of the front of card 5
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