Unit 1 biology cell fractionation

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Describe stage 1, homogensisation
Done by vibrating cells or blending them so the plasma membrane is broken up, releasing the organelles into the solution.
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Describe the solution it is kept in?
Cold, to reduce enzyme activity, isotonic, meaning the same chemical concentration as the cells being broken down, to prevent organelles bursting or shriveling due to the movement of water.
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Describe stage 2, Filtration.
The solution is filtered through a gauze to remove any debris, such as tissue or cell debris, the organelles in the solution are much smaller so pass through.
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In Untracetrifugation, what is the solution put in and what forms ifrst
The solution is poured into a tube and put in a centrifuge, where it is spun at a low speed. Heaviest organelles like nuclei are flung to the bottom, forming a thick sediment, while the rest of the organelles are suspended in the solution above.
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What is the solution above called?
the supernatant.
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What happens next?
the supernatant is drained of and put back again and again, spun faster and faster till all the organelles have flung to the bottom forming pellets.
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What is the order organelles are usually removed?
Nuclei, mitochondria, lysosomes, endoplasmic reticulum, ribosomes.
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Card 2

Front

Describe the solution it is kept in?

Back

Cold, to reduce enzyme activity, isotonic, meaning the same chemical concentration as the cells being broken down, to prevent organelles bursting or shriveling due to the movement of water.

Card 3

Front

Describe stage 2, Filtration.

Back

Preview of the front of card 3

Card 4

Front

In Untracetrifugation, what is the solution put in and what forms ifrst

Back

Preview of the front of card 4

Card 5

Front

What is the solution above called?

Back

Preview of the front of card 5
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