U2 ) Magnification + Light Microscope + Eyepiece Graticles 0.0 / 5 ? BiologyMicrobiology and fungiA2/A-levelAQA Created by: lieselb12Created on: 05-05-20 11:48 What is magnification? Number of times larger the image is compared to the actual size 1 of 13 What is resolution? Shortest distance at which 2 objects can be seen as two different objects. 2 of 13 For light microscopes the specimen must be...? Thin to allow light to pass through, can be alive or dead. 3 of 13 How much do eyepiece's magnify by? 10 4 of 13 What do objective lenses do? increase magnification furthur 5 of 13 What's the maximum magnification? x 1500 6 of 13 What is the maximum resolution? 0.2 um 7 of 13 What is a tempory mount? A slide to put the specimen on 8 of 13 How do you make a tempory mount? Add water to slide, cut thin specimen section, Add stain to specimen to increase contrast, use mounted needle to plase coverslip on 9 of 13 What are the properties of light microscopes? Images viewed in colour, low magnification + resolution, 2D images, cheap + not complicated to use. 10 of 13 How do you calculate magnification? Image size = Actual size x magnification . 11 of 13 How do you use an eyepiece graticle? 2 part 1 place micrometre slide(units) in microscope stage, calibrate(line up) with micrometre to work out worth of 1 division 12 of 13 How do you use an eyepiece graticle? 2 part 2 Replace micrometre with specimen slide + measure cell length + recalibrate at different magnifications 13 of 13
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