U2 ) Magnification + Light Microscope + Eyepiece Graticles

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  • Created by: lieselb12
  • Created on: 05-05-20 11:48
What is magnification?
Number of times larger the image is compared to the actual size
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What is resolution?
Shortest distance at which 2 objects can be seen as two different objects.
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For light microscopes the specimen must be...?
Thin to allow light to pass through, can be alive or dead.
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How much do eyepiece's magnify by?
10
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What do objective lenses do?
increase magnification furthur
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What's the maximum magnification?
x 1500
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What is the maximum resolution?
0.2 um
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What is a tempory mount?
A slide to put the specimen on
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How do you make a tempory mount?
Add water to slide, cut thin specimen section, Add stain to specimen to increase contrast, use mounted needle to plase coverslip on
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What are the properties of light microscopes?
Images viewed in colour, low magnification + resolution, 2D images, cheap + not complicated to use.
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How do you calculate magnification?
Image size = Actual size x magnification .
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How do you use an eyepiece graticle? 2 part 1
place micrometre slide(units) in microscope stage, calibrate(line up) with micrometre to work out worth of 1 division
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How do you use an eyepiece graticle? 2 part 2
Replace micrometre with specimen slide + measure cell length + recalibrate at different magnifications
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Other cards in this set

Card 2

Front

What is resolution?

Back

Shortest distance at which 2 objects can be seen as two different objects.

Card 3

Front

For light microscopes the specimen must be...?

Back

Preview of the front of card 3

Card 4

Front

How much do eyepiece's magnify by?

Back

Preview of the front of card 4

Card 5

Front

What do objective lenses do?

Back

Preview of the front of card 5
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