turbidimetry nephelometry

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from the intensity of light transmitted through the medium, the unscattered light is measured
turbidimetry
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from the intensity of light transmitted through the medium, the scattered light is measured
nephelometry
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the scattered light is measured at right angles to the incident beam
nephelometry
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Scattered intensity = K x initial intensity x concentration
nephelometry
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transmittance = intensity transmitted / initial intensity
turbidimetry
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the most common light source used in turbidimetry is
tungsten filament lamp
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direct measurement - measures the cloudiness of a solution by measuring the amount of light directly passing through a solution
turbidimetry
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indirect measurement - measures the amount of light scattered by the antigen antibody complexes
nephelometry
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glass cuvettes absorb ____
uv
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quartz cuvettes absorb
no radiation
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more sensitive
diode array
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a region in the molecule where the energy difference between two molecular orvitals falls within the coloured region of the em spectrum
chromophore
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more chromophores
compound absorbs at longer wavelength
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longer lifetime
phosphoescence
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when excited an emission obtained only for time of irratiation
fluorescence
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when excited an emission obtained for longer than time of irradiation
phosphoescence
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wavelength of fluorescence is of the wavelength of phosphoescence
smaller
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fluorescence _______ by oxygen/metals
quenched
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phosphorescence _____ by oxygen
completely inhibited
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Other cards in this set

Card 2

Front

from the intensity of light transmitted through the medium, the scattered light is measured

Back

nephelometry

Card 3

Front

the scattered light is measured at right angles to the incident beam

Back

Preview of the front of card 3

Card 4

Front

Scattered intensity = K x initial intensity x concentration

Back

Preview of the front of card 4

Card 5

Front

transmittance = intensity transmitted / initial intensity

Back

Preview of the front of card 5
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