Topic 6 Infection, Immunity and Forensics

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DNA is ...
non - overlapping
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What is a degenerate code?
containing more information than needed - some is redundant
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Gel Electrophoresis
A variation of chromatography in which DNA is seperated dependent on its mass and charge
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What does PCR stand for?
Polymerase Chain Reaction
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What does PCR do?
Increases the amount of DNA
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How can PCR be useful to DNA profiling
to make enough DNA to use in gel electrophoresis
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What is a primer?
small sequences of DNA which bind to the start of the seperated strands. It is needed in PCR to make enough DNA.
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What is the optimum temperature for DNA Polymerase?
75 degrees celsius
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What are the bonds between the bases in DNA?
Hydrogen bonds
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Difference between Introns and Exons
INTRONS are nonsense sections of DNA which dont make proteins while EXONS are sections of DNA which code for polypeptide chains
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What is an alternative name for the antisense strand?
Template strand
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Guve the defintion for RNA splicing?
The removal of introns from RNA and the joining of exons.
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Micro-satellite
2-4 bases of DNA repeated between 5-15 times
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Mini-satellite
20-50 bases sequence for DNA that will be repeated from 50 to several hundred times
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What is the name of the enzyme that is used to chop DNA into fragments?
Restriction endonucleases
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What cuts introns out of the pre-mRNA?
Spliceosomes
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Through what process can evidence for DNA profiling be presented in a courtroom?
Southern Blotting
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What is the role of free nucleotides?
They join on the complementary strands of DNA
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What is a difference between RNA and DNA?
In DNA there is Thymine and in RNA instead of Thymine there is Uracil
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Which organelle is involved in translation?
Ribosome
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What is the product of transcription and where does it leave through?
mRNA which leaves through the nuclear pores
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Card 2

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What is a degenerate code?

Back

containing more information than needed - some is redundant

Card 3

Front

Gel Electrophoresis

Back

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Card 4

Front

What does PCR stand for?

Back

Preview of the front of card 4

Card 5

Front

What does PCR do?

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