Studying genomes, DNA replication and Genetic Engineering Definitions
- Created by: une-meduse
- Created on: 07-06-13 12:15
Other questions in this quiz
2. PCR is different to natural replication as
- 1) pimer joins at 3' end 2) DNA polymerase attatches and adds free nucleotides 3) if a modified nucleotide added, polymerase thrown off 4) as reaction proceeds many molecules are made varying in size in each case the final nucleotide is marked
- 3) gel immersed in a buffer solution and electric current passed through for 2 hours 4) short lengths move faster than long lengths therefore move further 5) position of fragments can be shown with a dye or radioactive marker
- PCR can only replicate short sections of DNA, primer molecules must be added, heating and cooling is required
- 4) BACs taken and cultured, DNA extracted, restriction enzymes cut into smaller fragments 5) fragments sent through electrophoresis 5) sequenced using automated process 6) computers compare overlapping regions to reassemble whole BAC sequence
3. A vector can be
- Formed when DNA is cut using restriction enzymes, it is a short run of unpaired exposed bases seen at the end of the cut section, complimentary bases can anneal together as part of the process of recombining DNA fragments
- Bacterial plasmid, virus, yeast cell chromosomes
- It contains DNA that has been added to its cells as a result of genetic engineering
4. Somatic cell gene therapy involves
- Placing the gene into embryonic cell, this technique is not currently legal and is deemed unethical
- Placing of the gene in adult differentiated cells, examples include the placing of CFTR genes into the respiratory system cells of individuals with cystic fibrosis
- 1) improving a feature of the recipient cell eg resistant crops, growth controlling gene in farms 2) engineering organisms to synthesise useful products eg insulin, golden rice
5. The basic prodedure for electrophoresis is 1) DNA samples treated with restriction enzymes to split into fragments 2) placed in wells cut into the negative electrode end of gel
- 3) gel immersed in a buffer solution and electric current passed through for 2 hours 4) short lengths move faster than long lengths therefore move further 5) position of fragments can be shown with a dye or radioactive marker
- 1) pimer joins at 3' end 2) DNA polymerase attatches and adds free nucleotides 3) if a modified nucleotide added, polymerase thrown off 4) as reaction proceeds many molecules are made varying in size in each case the final nucleotide is marked
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