Studying cells - how microscope's work 0.0 / 5 ? BiologyCellular processes and structureASOCR Created by: JemmaVenessCreated on: 24-04-16 17:10 What is magnification? Size, how much bigger the image is than the specimen 1 of 15 What is resolution? Detail, how detailed the image is, how well a microscope distinguishes between two points that are close together 2 of 15 What is the calculation for magnification? Magnification = image size / object size 3 of 15 What are the three types of microscope? Light microscope, laser scanning confocal microscopes, electron microscopes 4 of 15 Describe light microscopes Use light, lower resolution, 0.2um, it is used to look at whole cells or tissues, the maximum magnification is about x1500 5 of 15 Describe laser scanning confocal microscopes Laser beams to scan a specimen with a fluorescent dye. The light from the fluorescent can be seen through a pin hole 6 of 15 What are the two types of electron microscope? Transmission electron microscope, Scanning electron microscope 7 of 15 Describe electron microscopes? Electromagnets to focus a beam of electrons through the specimen. 8 of 15 Describe scanning electron microscope Scan beam of electrons across specimen, 3D, lower resolution image 9 of 15 What are the maximum resolutions for all three? 0.2um, 0.0002um, 0.002um 10 of 15 What is the magnifications for all three? x1,500, more than x1,000,000 11 of 15 What can you do to stop a cell looking transparent? Stain your samples 12 of 15 How are staining samples made for light microscopes? Use a dye to help you see things ore clearly 13 of 15 Examples: Methlylene blue and eosin 14 of 15 How do you make a staining sample for electron microscopes? Objects are dipped in a solution of heavy metals 15 of 15
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