Studying cells - how microscope's work

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What is magnification?
Size, how much bigger the image is than the specimen
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What is resolution?
Detail, how detailed the image is, how well a microscope distinguishes between two points that are close together
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What is the calculation for magnification?
Magnification = image size / object size
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What are the three types of microscope?
Light microscope, laser scanning confocal microscopes, electron microscopes
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Describe light microscopes
Use light, lower resolution, 0.2um, it is used to look at whole cells or tissues, the maximum magnification is about x1500
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Describe laser scanning confocal microscopes
Laser beams to scan a specimen with a fluorescent dye. The light from the fluorescent can be seen through a pin hole
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What are the two types of electron microscope?
Transmission electron microscope, Scanning electron microscope
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Describe electron microscopes?
Electromagnets to focus a beam of electrons through the specimen.
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Describe scanning electron microscope
Scan beam of electrons across specimen, 3D, lower resolution image
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What are the maximum resolutions for all three?
0.2um, 0.0002um, 0.002um
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What is the magnifications for all three?
x1,500, more than x1,000,000
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What can you do to stop a cell looking transparent?
Stain your samples
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How are staining samples made for light microscopes?
Use a dye to help you see things ore clearly
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Examples:
Methlylene blue and eosin
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How do you make a staining sample for electron microscopes?
Objects are dipped in a solution of heavy metals
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Card 2

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What is resolution?

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Detail, how detailed the image is, how well a microscope distinguishes between two points that are close together

Card 3

Front

What is the calculation for magnification?

Back

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Card 4

Front

What are the three types of microscope?

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Card 5

Front

Describe light microscopes

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