RNA regulation continued

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What regulates genes in bacteria?
sRNA - Small RNA
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How long ae sRNAs?
between 80-110 bases in length/
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Bacterial sRNA are transcribed as..
small rna moleucles from their own promotor. they don't code for a protein.
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How many sRNA molecules does E.coli have. what percentage of the protien coding genes do they affect?
80 sRNAs, 2%
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How do sRNAs work, and what is their effect?
Base pairing with complimentary sequences within the target mRNA leading to - a. destruction, b.inhibition of translation or c. stimulation of translation
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Example - what does the sRNA oxyS bind to and what is it's effect?
it binds to flhA mRNA, which is a transcriptional activator of formate metabolism. oxyS switches off genes involved in formate metabolism
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Proteins known to cleave base pairing of sRNAs
RNAse E and RNase III endonucleases.
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Two domains of baterial RNase III
RNaselll domain - double stranded RNase activity. dsRBD - double stranded RNA binding domain
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Why did RNAi evolve?
to protect genomes from mutational effects of transposable genetic elements.
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Ways scientists are using rna interference to down regulate genes
to knock down expression of a specific gene to observe the effects of its absence. to create antiviral drugs, to create novel gene therapies, to develop anti-cancer drugs.
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an example of using antisense RNA in crops.
reduction of levels of polygalacturonase in tomatoes - PG catalyses cleavage of galacturonic acid residues in cell wall pectin.
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an example of using a siRNA to prevent viral infection
siRNA-based microbicide protects mice from lethal herpes simplex virus 2 infection
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An example of using siRNA to treat human diseases
Bevansiranib, siRNA that down regulates VegF. stops overgrowth of blood vessels behind the retina
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Card 2

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How long ae sRNAs?

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between 80-110 bases in length/

Card 3

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Bacterial sRNA are transcribed as..

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Preview of the front of card 3

Card 4

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How many sRNA molecules does E.coli have. what percentage of the protien coding genes do they affect?

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Card 5

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How do sRNAs work, and what is their effect?

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