Why is acid added to the root tips in the watch glass and why is this heated?
The acid causes the H - bonds between the DNA strands to break, allowing the stain to bind with some of the bases and therefore highlight the chromosomes against the rest of the cell. Heating increases the rate of this reaction.
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Why is a dye used?
To make the chromosomes visible.
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What is placed over the root tips, once they are on the microscope slide?
A cover slip.
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Why is the cover slip used to squash the root tips?
So that they are thin enough for light from the optical microscope to pass through them.
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Why is the root tip rinsed after being heated in the watch glass?
To remove the acid and prevent it from denaturing proteins inside the cell.
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Why is only vertical pressure applied to the cover slip?
To prevent the cells from sliding over each other, ensuring that they are visible through the microscope.
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Other cards in this set
Card 2
Front
Why is acid added to the root tips in the watch glass and why is this heated?
Back
The acid causes the H - bonds between the DNA strands to break, allowing the stain to bind with some of the bases and therefore highlight the chromosomes against the rest of the cell. Heating increases the rate of this reaction.
Card 3
Front
Why is a dye used?
Back
Card 4
Front
What is placed over the root tips, once they are on the microscope slide?
Back
Card 5
Front
Why is the cover slip used to squash the root tips?
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