Mitosos - Cloning Using Tissue Culture (Procedure + Reason)

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  • Created by: Nicole D
  • Created on: 30-10-13 10:12
Cloning Using Tissue Culture
Procedure and Reason
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The tips of the stems and side shoots are removed from the plant to be cloned. These parts are called explants.
Contain the vital auxin hormone.
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Sterilise explants
Removes bacteria
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Place explants in an agar medium containing nutrients and hormones to encourage cell division and callus (start of new plant) formation.
To encourage cell division and so the plant grows.
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Using a different mixture of hormones, stimulate clumps of cells to form shoots.
So cells differentiate into shoot cells.
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Explants with shoots transferred to another culture medium containg a different balance of hormones to induce root formation.
Encourage differentiation of cells to form roots.
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Plants transferred to greenhouses and transplanted into compost.
So plant is encouraged to grow and so the soil / compost produces nutrients.
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Other cards in this set

Card 2

Front

Contain the vital auxin hormone.

Back

The tips of the stems and side shoots are removed from the plant to be cloned. These parts are called explants.

Card 3

Front

Removes bacteria

Back

Preview of the back of card 3

Card 4

Front

To encourage cell division and so the plant grows.

Back

Preview of the back of card 4

Card 5

Front

So cells differentiate into shoot cells.

Back

Preview of the back of card 5
View more cards

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