Microscopy Revision

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  • Created by: ElishaG
  • Created on: 20-02-17 17:10
What is magnification?
The number of times larger the image is compared to the object.
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What is resolution?
The ability to see two objects that are close together as separate objects to see detail.
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What are the three types of microscope?
1)Light microscope. 2)Transmission electron microscope(TEM). 3)Scanning electron microscope(SEM).
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What is the magnification and resolution of a light microscope?
Magnification= x1500. Resolution= 200nm.
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What is the magnification and resolution of a TEM?
Magnification= x500,000 Resolution= 0.1-0.4nm
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What is the magnification and resolution of an SEM?
Magnification= x100,000 Resolution=0.1-0.4nm
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What are stains?
Coloured chemicals that bind to the specimen.
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Why is staining useful when using a microscope?
1)Features become visible and can be seen.2)Increases contrast.3)Allows identification of organelles.4)Allows identification of different molecules.
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What are the advantages of a light microscope?
1)Can see both dead and living things. 2)Can use stains to make things visible. 3)Don't need high level training/skill to use.
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What are disadvantages of a light microscope?
1)Can't see organelles. 2)Low resolution. 3)Low magnification,
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What are the advantages of electron microscopes?
1)High magnification. 2)High resolution. 3)3D surface detail (SEM only) 4)Can see ultra structure (TEM).
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What are disadvantages of electron microscopes?
1)Artefacts.2)Black and white only. 3)Can only see dead things. 4)Have to be highly skilled to use it.
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Put these units if measurement in the correct order: Millimetre, nanometre, micrometre, metre.
Metre, Millimetre, Micrometre, Nanometre.
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What is the formula for magnification?
Measured length/scale bar label.
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What is the formula for actual size?
Picture size/magnification.
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Other cards in this set

Card 2

Front

What is resolution?

Back

The ability to see two objects that are close together as separate objects to see detail.

Card 3

Front

What are the three types of microscope?

Back

Preview of the front of card 3

Card 4

Front

What is the magnification and resolution of a light microscope?

Back

Preview of the front of card 4

Card 5

Front

What is the magnification and resolution of a TEM?

Back

Preview of the front of card 5
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