Microscopy Revision 0.0 / 5 ? BiologyMicroscopyA2/A-levelOCR Created by: ElishaGCreated on: 20-02-17 17:10 What is magnification? The number of times larger the image is compared to the object. 1 of 15 What is resolution? The ability to see two objects that are close together as separate objects to see detail. 2 of 15 What are the three types of microscope? 1)Light microscope. 2)Transmission electron microscope(TEM). 3)Scanning electron microscope(SEM). 3 of 15 What is the magnification and resolution of a light microscope? Magnification= x1500. Resolution= 200nm. 4 of 15 What is the magnification and resolution of a TEM? Magnification= x500,000 Resolution= 0.1-0.4nm 5 of 15 What is the magnification and resolution of an SEM? Magnification= x100,000 Resolution=0.1-0.4nm 6 of 15 What are stains? Coloured chemicals that bind to the specimen. 7 of 15 Why is staining useful when using a microscope? 1)Features become visible and can be seen.2)Increases contrast.3)Allows identification of organelles.4)Allows identification of different molecules. 8 of 15 What are the advantages of a light microscope? 1)Can see both dead and living things. 2)Can use stains to make things visible. 3)Don't need high level training/skill to use. 9 of 15 What are disadvantages of a light microscope? 1)Can't see organelles. 2)Low resolution. 3)Low magnification, 10 of 15 What are the advantages of electron microscopes? 1)High magnification. 2)High resolution. 3)3D surface detail (SEM only) 4)Can see ultra structure (TEM). 11 of 15 What are disadvantages of electron microscopes? 1)Artefacts.2)Black and white only. 3)Can only see dead things. 4)Have to be highly skilled to use it. 12 of 15 Put these units if measurement in the correct order: Millimetre, nanometre, micrometre, metre. Metre, Millimetre, Micrometre, Nanometre. 13 of 15 What is the formula for magnification? Measured length/scale bar label. 14 of 15 What is the formula for actual size? Picture size/magnification. 15 of 15
Comments
No comments have yet been made