Microscopy

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What is magnification?
Magnification tells us how many times larger an image seen through a microscope is compared to the real object.
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What is resolution?
Resolution is the ability to distinguish between (tell apart) two or more objects that are close together
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Whats the equation for magnification?
Magnification = image size / actual size
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A cell has a diameter of 0.001cm. An image of the cell viewed through the microscope has a diameter of 5 cm. The magnification is hence:
5 cm/0.001 cm = x5000.
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What is a light microscope?
A light microscope passes light through a specimen, and creates a magnified image using lenses. The first light microscope was made using two lenses in the late 16th century. It had a resolution better than the human eye itself
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Name a discovery made using the light microscope
Light microscopes allowed bacteria to be seen for the first time. They also allowed us to see plant cells and animal cells as separate objects. However, we still could not tell lots of sub-cellular (within the cell) structures apart.
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What is an electron micriscope?
The electron microscope was first used in 1933. Electron microscopes work in a similar way to light microscopes. However, electrons are passed through the specimen instead of light. They can now resolve distances of 1nm (better resolution) with magni
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Name discoveries made using the electron microscope
The high level of detail of electron microscopes allowed scientists to see sub-cellular structures more clearly. They also allowed scientists to study how structures such as mitochondria, chloroplasts and ribosomes function.
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How do you perform a Light Microscope experiment?
1 Put a thin sample of tissue onto a slide. 2 Add a few drops of a suitable dye. 3 Place a coverslip on top of the tissue and place the slide onto the microscope stage. 4 Use the objective lens with the lowest magnification and focus on the sample
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Other cards in this set

Card 2

Front

What is resolution?

Back

Resolution is the ability to distinguish between (tell apart) two or more objects that are close together

Card 3

Front

Whats the equation for magnification?

Back

Preview of the front of card 3

Card 4

Front

A cell has a diameter of 0.001cm. An image of the cell viewed through the microscope has a diameter of 5 cm. The magnification is hence:

Back

Preview of the front of card 4

Card 5

Front

What is a light microscope?

Back

Preview of the front of card 5
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