They work by passing light through the specimen. Thye let us see things such as nuclei and chloroplasts and we can use the to study living cells
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When were the electro miscroscopes invented?
1930s
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What increases when you use an electron microsope?
Nagnification and resolution
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What is step 1 to view a specimen using a light microscope?
Get a thin layer to allow light through
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What is step 2 to view a specimen using a light microscope?
Take a clean slide and put a drop of water on the slide to secure the specimen. then with tweezers place the specimen on the slide
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What is step 3 to veiw a specimen using a light microscope?
Add a drop of stain if your specimen is completely transparent or colourless
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What is step 4 to veiw a specimen using a light microscope?
Place a cover slip over the top
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What is step 5 to veiw a specimen using a light microscope?
Select the lowest-powered objective lens
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What is step 6 to veiw a specimen using a light microscope?
use a coarse adjustment knob to move the stage just underneath the objective lens. Then, looking through the eyepiece, move the stage downwards until the specimen is nearly in focus
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What is step 7 to veiw a specimen using a light microscope?
Adjust the focus with the fine adjustment knob, until you get a clear image.
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What is step 8 to veiw a specimen using a light microscope?
If you need to see you specimen wiht greater maginifiction, swap to a higher-powered objective lens, refocus and recalculate teh FOV accordingly
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What is the first step to creting a scientific drawing of a specimen?
Using a sharp pencil, draw outlines of the main features using clear, unbroken lines. NEVER INCLUDE ANY COLOURING OR SHADING!!!
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What is the second step?
Make sure th edrawing takes uip at aleast half of the space available and remember to keep all the parts in proportion
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What is the third and final step?
Label the important features with striaght lines that don't cross over. Include the maganification used and a scale.
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What is magnification?
Magnification = how many times bigger the image is
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What is the formula for total magnification?
total magnificaation = eyepiece lens magnification X objective lens magnification
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What is the formula for magnification?
magnification = image size / real size
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What is millimetre in standard form?
x 10-3m
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what is micrometre in standard form?
x 10-6
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What is nanometre in standard form?
x 10-9
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What is picometre in standard form?
x 10-12
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Calculate the length of the cell which has an image size of 7 x 10-1mm under magnification of x 400. write you answer in micrometre.
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