Microscopy

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How many lenses does a compound light microscope have? What are they called?
2 lenses - the objective lense which goes near the specimen and the eyepiece lense which you view the specimen through
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What is the calculation for magnification?
Magnification = Size of image / actual size of object
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How do you convert mm into nm?
Multiply the mm by 1,000,000
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What is a TEM?
Transmission electron microscope
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What is an SEM?
Scanning electron microscope
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What is the resolving power of a TEM?
0.5nm (Has the best resolution)
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What is the resolving power of a SEM?
3 - 10nm (Not as good resolution-wise but produces great 3D images)
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List some advantages of a light microscope
Inexpensive to buy and operate, small and portable, simple sample preparation, sample preparation does not usually lead to distortion, vacuum is not required, natural colour of samples is seen, specimens can be living or dead
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List some disadvantages of an electron microscope
Expensive to buy and operate, large and needs to be installed, complex sample preparation, sample preparation often distorts material, vacuum is required, specimens must be dead
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What is an artefact? Give an example of one
A visible structural detail caused by processing the specimen and not a feature of the specimen. E.G; An air bubble under the cover slip
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Other cards in this set

Card 2

Front

What is the calculation for magnification?

Back

Magnification = Size of image / actual size of object

Card 3

Front

How do you convert mm into nm?

Back

Preview of the front of card 3

Card 4

Front

What is a TEM?

Back

Preview of the front of card 4

Card 5

Front

What is an SEM?

Back

Preview of the front of card 5
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