Microbiology lecture 5

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What is the turbidity method? Is it destructive and continuous?
Measures light scattering by cells - spectrophotometer. It is non-destructive and continuous
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What are the disadvantages of the turbidity method?
Measure all particles i.e.. dead cells. Low sensitivity
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What is flow cytometry and FACs?
Measure particles in a microfluidic flow. Highly automated. Can measure fluorescence at multiple wavelengths. FACs - fluorescence activated cell sorting
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What is the first stage of the bacterial growth cycle?
Lag phase - cells adjust to new conditions
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What is the second stage of the bacterial growth cycle?
Exponential phase - optional growth with regular doubling in cell no.
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What is the third stage of the bacterial growth cycle?
Stationary phase - growth limited by nutrient depletion or accumulation of toxic metabolites
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What is the fourth stage of the bacterial growth cycle?
Death phase - complex gradual loss of viability but with some cell turnover
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Where does bacterial chromosome replication start? and what direction does it go in?
From oriC and bidirectionally. Replication is initiated in the previous cycle
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What is bacterial chromosome replication controlled by?
DnaA - recruits replisome
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Where does bacterial chromosome replication stop?
terC
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Name the two spatial cues in cell division...
1) The Min system goes to cell poles and inhibits division there 2) The Nucleoid Occlusion system inhibits division in the vicinity of the nucleoid
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What is a divisome?
FtsZ (tubulin). Assembles into ring like structure - 'z-ring'
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Card 2

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What are the disadvantages of the turbidity method?

Back

Measure all particles i.e.. dead cells. Low sensitivity

Card 3

Front

What is flow cytometry and FACs?

Back

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Card 4

Front

What is the first stage of the bacterial growth cycle?

Back

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Card 5

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What is the second stage of the bacterial growth cycle?

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