Microbiology lecture 3

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Explain the Gram staining method...(first two steps)
1) Flood smear with CRYSTAL VIOLET for 1 MINUTE. All cells = PURPLE 2) Add IODINE solution for 1 MINUTE. All cells remain PURPLE.
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Explain Gram staining method...(last two steps)
3) DECOLOURIZE with ALCOHOL briefly about 20 SECONDS. Gram +ve cells = PURPLE and Gram -ve cells = COLOURLESS 4) Counterstain with SAFRANIN for 1-2 MINUTES. Gram +ve = PURPLE, Gram -ve = PINK --> RED
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What do cells have to be to be seen on an electron microscope?
Fixed, dehydrated, embedded, sectioned and staining.
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What do cells have to be to be seen on an cryoelectron microscopy and tomography?
Flash frozen in live state.
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What does a cryoelectron microscopy and tomography produce?
3D reconstruction, contrast poor
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Card 2

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Explain Gram staining method...(last two steps)

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3) DECOLOURIZE with ALCOHOL briefly about 20 SECONDS. Gram +ve cells = PURPLE and Gram -ve cells = COLOURLESS 4) Counterstain with SAFRANIN for 1-2 MINUTES. Gram +ve = PURPLE, Gram -ve = PINK --> RED

Card 3

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What do cells have to be to be seen on an electron microscope?

Back

Preview of the front of card 3

Card 4

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What do cells have to be to be seen on an cryoelectron microscopy and tomography?

Back

Preview of the front of card 4

Card 5

Front

What does a cryoelectron microscopy and tomography produce?

Back

Preview of the front of card 5

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