Microbiology lecture 3 0.0 / 5 ? BiologyBacterial shape, size and their appendagesUniversityNone Created by: willmiddlehamCreated on: 02-04-15 14:42 Explain the Gram staining method...(first two steps) 1) Flood smear with CRYSTAL VIOLET for 1 MINUTE. All cells = PURPLE 2) Add IODINE solution for 1 MINUTE. All cells remain PURPLE. 1 of 5 Explain Gram staining method...(last two steps) 3) DECOLOURIZE with ALCOHOL briefly about 20 SECONDS. Gram +ve cells = PURPLE and Gram -ve cells = COLOURLESS 4) Counterstain with SAFRANIN for 1-2 MINUTES. Gram +ve = PURPLE, Gram -ve = PINK --> RED 2 of 5 What do cells have to be to be seen on an electron microscope? Fixed, dehydrated, embedded, sectioned and staining. 3 of 5 What do cells have to be to be seen on an cryoelectron microscopy and tomography? Flash frozen in live state. 4 of 5 What does a cryoelectron microscopy and tomography produce? 3D reconstruction, contrast poor 5 of 5
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