Methods 0.0 / 5 ? ScienceImpact of pH on amylaseA2/A-levelAQA Created by: hannahgnikCreated on: 07-01-22 12:40 Step 1: Label a test tube with pH to be tested 1 of 12 Step 2: Use a syringe to place 1cm3 of amylase into the test tube 2 of 12 Step 3: Use a different syringe to place 2cm3 of starch into a different test tube 3 of 12 Step 4: Add 1cm3 of the pH buffer solution to the test tube containing amylase using a new syringe 4 of 12 Step 5: Place both of the test tubes into a 37 degree water bath and leave for 5 minutes 5 of 12 Step 6: Add 2 drops of iodine to each well of a spotting tile 6 of 12 Step 7: Add 1 drop of starch into the first well to act as a control 7 of 12 Step 8: Once the temperatures have equilibrated mix the test tubes. Start the stop watch and add a drop from the mixture to a new well every 20 seconds 8 of 12 Step 9: Repeat step 8 until the iodine solution and the amylase/buffer/starch mixture remain orange. Record this time 9 of 12 Step 10: Repeat this procedure 3 times 10 of 12 Step 11: Repeat the whole procedure with another of the pH buffers to be used, or pool the class results 11 of 12 Step 12: Plot a graph of time taken to break down starch against pH, or calculate the rate of reaction and plot rate against pH 12 of 12
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