1. Prepare sucrose solutions (serial dilution) 2. Cut potato into chips (exactly the same size) remove skin, pat dry, weigh 3. Put in sucrose solutions separately and leave for at least 1 hour (bung on) 4. Then remove, dry, weigh find % change
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Investigating osmosis in articical cells
1. Prepare sucrose solutions (serial dilution) 2. Using viscous tubing and pipette 0.4 sucrose solution in and tie knot/ paperclip 3. Weigh then place in the various sucrose concentrations 4. After 20 minutes remove then reweigh, calculate % change
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Investigating effect of temperature on membrane permeability
1. 10cm3 distilled water in thermostatically controlled water baths 2. Cut, rinse, dry beetroot then add to distilled water, leave for 15 mins 3. Remove beetroot then pour liquid into cuvette 4. Measure absorption using green filter
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Why does Beetroot pigment escape?
As temp of the beetroot cells increase there is more kinetic energy meaning the molecules in the membrane move around more which increases it's fluidity and denatures the proteins meaning there are gaps in the membrane so betalain can escape
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What would happen if ethanol was used in beetroot experiment
Ethanol breaks bonds in proteins and denatures so disintegrates membrane so that there are gaps
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Is viscous tubing comparable to actual cells?
Yes because it allows substances in and out however does not contain proteins and is not a phospholipid bilayer
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Whats the purpose of placing bungs in test/boiling tubes?
Prevents evaporation
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Define amphipathic
When a molecule has a hydrophobic and hydrophobic region
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Other cards in this set
Card 2
Front
1. Prepare sucrose solutions (serial dilution) 2. Using viscous tubing and pipette 0.4 sucrose solution in and tie knot/ paperclip 3. Weigh then place in the various sucrose concentrations 4. After 20 minutes remove then reweigh, calculate % change
Back
Investigating osmosis in articical cells
Card 3
Front
1. 10cm3 distilled water in thermostatically controlled water baths 2. Cut, rinse, dry beetroot then add to distilled water, leave for 15 mins 3. Remove beetroot then pour liquid into cuvette 4. Measure absorption using green filter
Back
Card 4
Front
As temp of the beetroot cells increase there is more kinetic energy meaning the molecules in the membrane move around more which increases it's fluidity and denatures the proteins meaning there are gaps in the membrane so betalain can escape
Back
Card 5
Front
Ethanol breaks bonds in proteins and denatures so disintegrates membrane so that there are gaps
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