Making lipids (triacylglycerol)

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Describe elongation of lipids
2 carbons added at a time, the carbons are from Malonyl CoA, (Enzyme: Elongases)
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Describe unsaturation of fatty acids
Introduction of double bonds, oxidative reaction, requires NADH or NADPH, (Enzyme: Fatty acid acyl-CoA desaturase)
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What are triacylglycerols compromised of?
A glycerol molecule and 3 fatty acids
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What are the 2 stages of making a triacylglycerol?
1) Making glycerol-3-phosphate (G3P), 2) Create ester linkages between fatty acids and the O in the glycerol backbone
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What type of process is this?
Sequential- one fatty acid is added at a time
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What is G3P?
The "activated" form of glycerol
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What are the 2 route to making G3P?
1) Phosphorylating glycerol, 2) Reducing dihydroxyacetone phosphate (DHAP) -- Both require energy--
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Describe how you make G3P from glycerol
Phosphoryl group from 1 ATP molecule is attached to C3 of a glycerol molecule, (Enzyme: Glycerol Kinase)
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Describe how you make G3P from dihydroxyacetone
DHAP is reduced to form G3P, hydrogen donor NADH+H, (Enzyme: Glycerol-3-phosphate dehydrogenase), reversible, DHAP from glycolysis
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Once G3P is synthesised, what happens?
Fatty acids must be converted to the corresponding acyl CoAs, energy requiring process- 2 ATP per fatty acid, (Enzyme: Fatty acyl CoA synthase)
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What is the first step of the second stage? (after the fatty acids have been converted)
The fatty acyl group of the first fatty acyl CoA is esterified at the -OH from C1 of the G3P, (Enzyme: Glycerol-3-phosphate acyltransferase), product: Lysophosphatidic acid
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What is the second step?
The 2nd fatty acyl CoA is esterified at the _OH of C2 of the lysophosphatidic acid, (Enzyme: Glycerol-3-phosphate acyltransferase), product: Phosphatidic acid (A glycerophospholipid)
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What is the third step?
The phosphate group from the C3 is removed (Enzyme: phosphatidate phosphatase), product: Diacylglycerol
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What is the final step?
A final fatty acyl CoA is esterified on C3 of DAG, (Enzyme: acyltransferase), product: Triacylglycerol
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Other cards in this set

Card 2

Front

Describe unsaturation of fatty acids

Back

Introduction of double bonds, oxidative reaction, requires NADH or NADPH, (Enzyme: Fatty acid acyl-CoA desaturase)

Card 3

Front

What are triacylglycerols compromised of?

Back

Preview of the front of card 3

Card 4

Front

What are the 2 stages of making a triacylglycerol?

Back

Preview of the front of card 4

Card 5

Front

What type of process is this?

Back

Preview of the front of card 5
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