1.1 (1) Living organisms consist of cells

Define magnification
Magnification is the degree to which the size of an image is larger than the object itself.
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Define resolution
Resolution refers to the ability to see two distinct points separetley.
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What is the magnification and resolution of a light microscope?
magnification = x1500 Resolution = 200nm
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Why is the resolution only 200nm?
Because the particles have to be atleast half the wavelength of light apart to see them with a light microscope.
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What are the two methods of preparation of specimens for a light microscope?
Staining and sectioning
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Explain staining.
Coloured stains are chemicals the bind to chemicals on or in the specimen. Some stains bing to specific cell structures.
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Give two examples of stains that bind to specific cell structures.
Acetic orcien stains DNA dark red, gentian violet stains bacterial cell walls.
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Explain sectioning.
Specimens are embedded in wax, then thin sections are cut without distorting the shape of the specimen.
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Card 2

Front

Define resolution

Back

Resolution refers to the ability to see two distinct points separetley.

Card 3

Front

What is the magnification and resolution of a light microscope?

Back

Preview of the front of card 3

Card 4

Front

Why is the resolution only 200nm?

Back

Preview of the front of card 4

Card 5

Front

What are the two methods of preparation of specimens for a light microscope?

Back

Preview of the front of card 5
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