Light microscope, resolution + magnification

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  • Created by: Steff06
  • Created on: 29-03-16 11:31
What is the definition of resolution?
The ability to distinguish 2 separate points as distinct from each other.
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What is the definition of magnification?
The number of times greater an image is than the object.
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How does a light microscope work?
Light passes through a bulb under the stage, through a condenser lens then through the specimen. Beam of light is focused through the objective lens then through the eyepiece lens.
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What is the magnification and resolution of a light microscope?
Magnification = x1500. Resolution = 200nm
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What is an issue with light microscopes?
The resolution is not high so cannot give detailed information about internal cell structure.
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What is the purpose of staining?
To provide contrast between organelles and to allow them to be identified. To highlight specific organelles/areas.
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How is staining done?
Chemicals bind to chemicals or on the specimen. Some stains bind to specific cell structures e.g. Acetic orcein binds to DNA and gentian violent binds to bacterial cell walls.
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Why is sectioning used?
To allow sections of organisms to be viewed without distorting the cell structure.
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How is sectioning done?
Specimens are embedded in wax and thin sections are then cut.
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What is sectioning especially useful for?
Useful for making sections of soft tissue e.g. the brain.
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Card 2

Front

What is the definition of magnification?

Back

The number of times greater an image is than the object.

Card 3

Front

How does a light microscope work?

Back

Preview of the front of card 3

Card 4

Front

What is the magnification and resolution of a light microscope?

Back

Preview of the front of card 4

Card 5

Front

What is an issue with light microscopes?

Back

Preview of the front of card 5
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