Investigating Enzyme Action (2.1.3)

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What is the independent variable?
The thing you alter.
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What is the dependent variable?
The thing that you measure.
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What is equilibration?
Placing enzyme and substrate separately in a water bath so that they reach the required temperature before the investigation begins.
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How can temperature be kept constant?
Carry out experiment in a water bath with a thermostat.
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How can enzyme concentration be kept constant?
If using an enzyme in living tissue, accurately measure mass.
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How can substrate concentration be kept constant?
Use accurately measured volume or mass of substrate.
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How can pH be kept constant?
Use pH buffers.
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What are the two ways to measure reaction rate?
Measure the amount of product made after a fixed period of time. Measure how much substrate has been used up after a fixed period of time.
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What is the equation for reaction rate?
Reaction rate= 1/time
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Card 2

Front

What is the dependent variable?

Back

The thing that you measure.

Card 3

Front

What is equilibration?

Back

Preview of the front of card 3

Card 4

Front

How can temperature be kept constant?

Back

Preview of the front of card 4

Card 5

Front

How can enzyme concentration be kept constant?

Back

Preview of the front of card 5
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