genomes and gene technology to accompany powere point :) enojy and good luck :) 4.5 / 5 based on 8 ratings ? BiologyDNA, genetics and evolutionA2/A-levelOCR Created by: danni :)Created on: 22-05-13 08:29 one gene technology is polymerase chain reaction (PCR) 1 of 15 if a whole genome needs sequencing, you should use PCR 2 of 15 the first three steps of PCR are: DNA is denatured by heating to 95˚C The mixture is then cooled to between 50˚C - 65˚C The mixture is then heated to 72˚C 3 of 15 during electrophoresis A direct current is passed through the gel and the DNA fragments (which carry a small negative charge) are attracted to the anode (positive electrode) 4 of 15 restriction enzymes cut DNA into pieces with sticky ends 5 of 15 DNA ligase Catalyses condensation reaction that joins the phosphate- sugar backbones in the helix together 6 of 15 an ethical issue with genetic engineering is using antibiotic resistant genes as markers may increase number of antibiotic resistant pathogens in our environment 7 of 15 what type of diabetes do people who have to inject themselves with insulin have? type 1 8 of 15 xenotransplantation is the transplantation of cells, tissues or organs from one species to another 9 of 15 gene therapy involves altering alleles 10 of 15 an advantage of gene therapy is: it prolongs lives of people with genetic disorders 11 of 15 DNA probes can see if DNA contains mutated gene 12 of 15 chain termination determines order of bases in a section of DNA 13 of 15 BAC stands for bacterial artificial chromosome 14 of 15 comparing genomes of different species allows us to: Understand evolutionary relationships. Closely related species will share more DNA 15 of 15
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