genomes and gene technology

to accompany powere point :) enojy and good luck :)

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  • Created by: danni :)
  • Created on: 22-05-13 08:29
one gene technology is
polymerase chain reaction (PCR)
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if a whole genome needs sequencing, you should use
PCR
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the first three steps of PCR are:
DNA is denatured by heating to 95˚C The mixture is then cooled to between 50˚C - 65˚C The mixture is then heated to 72˚C
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during electrophoresis
A direct current is passed through the gel and the DNA fragments (which carry a small negative charge) are attracted to the anode (positive electrode)
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restriction enzymes cut DNA into pieces with
sticky ends
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DNA ligase
Catalyses condensation reaction that joins the phosphate- sugar backbones in the helix together
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an ethical issue with genetic engineering is
using antibiotic resistant genes as markers may increase number of antibiotic resistant pathogens in our environment
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what type of diabetes do people who have to inject themselves with insulin have?
type 1
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xenotransplantation is
the transplantation of cells, tissues or organs from one species to another
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gene therapy involves
altering alleles
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an advantage of gene therapy is:
it prolongs lives of people with genetic disorders
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DNA probes can
see if DNA contains mutated gene
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chain termination
determines order of bases in a section of DNA
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BAC stands for
bacterial artificial chromosome
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comparing genomes of different species allows us to:
Understand evolutionary relationships. Closely related species will share more DNA
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Other cards in this set

Card 2

Front

if a whole genome needs sequencing, you should use

Back

PCR

Card 3

Front

the first three steps of PCR are:

Back

Preview of the front of card 3

Card 4

Front

during electrophoresis

Back

Preview of the front of card 4

Card 5

Front

restriction enzymes cut DNA into pieces with

Back

Preview of the front of card 5
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