genetic fingerprinting

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  • Created by: emma
  • Created on: 22-04-13 13:55
what is the first stage of genetic fingerprinting?
extracting the DNA by seperating it from the rest of the cell, then multiplying it by PCR.
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what is the second stage of genetic fingerprinting?
Digestion- the DNA is cut into fragments by restriction endonucleases which cut close to but not within groups of core sequences
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how are the DNA fragments seperated in stage 3?
by gel electrophoresis
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why is the gel immersed in alkali?
to seperate the double strands into single strands
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what are the stages of southern blotting?
the process by which single stransa are transfered onto a nylon membrane. 1)nylon membrane over gel 2)cover with absorbent paper(draws up liquid containing DNA by capillary action) 3) DNA transfered onto nylon in same places 4) UV fixes fragments
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what is hybridisation?
radioactive/fluorescent probes bind to core sequences. (they have base sequences complementary to the core sequences). this is carried out with different probes, each of which binds to a different core sequence.
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what is the final stage of DNA fingerprinting?
x-ray film is placed over nylon membrane and is exposed by radiation from the radioactive probes. series of bars unique to each individual is produced.
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Card 2

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what is the second stage of genetic fingerprinting?

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Digestion- the DNA is cut into fragments by restriction endonucleases which cut close to but not within groups of core sequences

Card 3

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how are the DNA fragments seperated in stage 3?

Back

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Card 4

Front

why is the gel immersed in alkali?

Back

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Card 5

Front

what are the stages of southern blotting?

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