Genetic Modification 0.0 / 5 ? BiologyGenetic ModificationGCSEEdexcel Created by: RinkyPereraCreated on: 22-02-17 20:52 What is genetic engineering? The cutting of a useful gene out of one organism and pasting it into another 1 of 14 What is a plasmid? A loop of DNA 2 of 14 Bacteria that contain the gene for human insulin are... ...transgenic 3 of 14 What is Agrobacterium tumefaciens? It is a pathogen that invades plant cells and inserts its genes into the plant's DNA 4 of 14 How do we make insulin? When the human insulin gene can be stuck into bacteria so that they make insulin 5 of 14 Step number 1 for engineering bacteria to produce insulin... ...a plasmid is removed from a bacterium 6 of 14 Step number 2... ...the insulin gene is cut out of a human chromosome using a restriction enzyme. 7 of 14 Step number 3... ...the plasmid is cut open using the same restriction enzyme leaving the same sticky ends 8 of 14 Step number 4... ...the plasmid and the human insulin gene are mixed together 9 of 14 Step number 5... ...ligase (enzyme) is added. This joins the sticky ends together to produce recombinant DNA 10 of 14 Step number 6... ...the recombinant DNA is inserted into a bacterium 11 of 14 Step number 7... ...the modified bacterium is grown in a fermenter 12 of 14 What will I do now after you said all the steps correctly? APPLAUD!!!! 13 of 14 What will I do if you didn't say all the steps correctly? Tell you to go back and check it again!!!! 14 of 14
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