FM - Monitoring Bacteria

1. What does Crystal Violet do in gram staining?
Dye dissociates into CV^+ and Cl^- .....

• These are taken up by negative cell types where the Cl^- ions bind and stain the negatively charged components of the bacterial cell wall. Only gram -ve cells are purple.
• These are taken up by both cell types where the CV ^+ ions bind and stain the negatively charged components of the bacterial cell wall. Both gram +ve and -ve cells are purple.
• These are taken up by positive cell types where the CV^+ ions bind and stain the negatively charged components of the bacterial cell wall. Only gram +ve cells are purple.