Exploiters pt 3

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  • Created by: Tabitha
  • Created on: 21-03-15 10:46
Explain how to make a wet mount
Two drops of sample, add cover slip, seal with vaseline, examine
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Explain how to make a dry mount
1 loopful of water, take small amount of sample of loop from colony, mix, dry with bunsen
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Procedures in Crystal violet stain
Cover in stain for 20 sec, wash with water, blot dry
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Give 4 lab rules
Always wear lab coat, wash hands on leaving, don't eat/drink, use aseptic technique
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Give symbol and conversion between millimetre and micrometre
mm and um 1mm=1000um
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Name 3 smaller measurements that micrometre
nanometre (nm), Angstrom (A), pictometre (pm)
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Name the samples observed in the practical
Hay infusion pond water, spoiled fruit, growth on N-free agar, agar plates given
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Name 3 microscopes
Vickers, Leitz and Olympus
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Name the turning bit with10-x100 maginifcation
Objective lens
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How to use the stage and why
Raise as high as possible, focus by lowering, done so unlikely to crack slide
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Give two facts about lenses
Don't touch lens with fingers, always use immersion oil with x100
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What was found on given agar plates?
65B Clusters (Straphlycoccus) Gram+, 16M bacillus (Ecoli) Gram -ve
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Describe fungal hyphae
Branching and filamentous, can be coenocytic (one) or divided into cells by septa
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Name 2 spore arrangements
Sporangiospores in a sproangium or conidiospores arranged in chains at end on conidiophore
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4 common algal forms
Microasterias (Wide and flat), Volvox (circular), Scenedesmus (Stacks of 4), Spirogyra (Blobs in tubes)
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Describe Lactobacillus
Rod shaped, can be in chains
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Describe staphylcoccus
spherical in random or grape like clusters
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Describe streptococcus
Chains of cocci
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Example of common Protozoan
Paramoecium (Ciliated protozoan) Contains Nucleus, cilla, and contractile vacuole
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Other cards in this set

Card 2

Front

Explain how to make a dry mount

Back

1 loopful of water, take small amount of sample of loop from colony, mix, dry with bunsen

Card 3

Front

Procedures in Crystal violet stain

Back

Preview of the front of card 3

Card 4

Front

Give 4 lab rules

Back

Preview of the front of card 4

Card 5

Front

Give symbol and conversion between millimetre and micrometre

Back

Preview of the front of card 5
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