enzyme substrate

change
concentration of enzyme
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enzyme
Type of enzymes the same
2 of 8
reliability
Repeat 4 times, calculate mean, discard anomalies
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measurement
Vol. of gas produced. In seconds. RATE - divide the volume by the number of seconds
4 of 8
same
Volume of distilled water, volume of enzyme, volume of subsrate, concentration of subsrate, PH, temperature
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safety
Wear eye protection, lab coats and disposable gloves.All enzymes are potential allergens and skin contact should be avoiced. Any sipllages onto the skin should be washed off immediatly. Asthma sufferers may be particularly sensitive. Hydrogen peroxid
6 of 8
controls
Maintaining constant temperature Accurately making up the different concentrations Identifying end point consistently Difficult to see the cross through the solution - if doing qualitatively vs quantitatively.
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method
Add 2cm3 of yeast to one test tube. Add 4cm3 of hydrogen peroxide solution at a concentration of 20% to the other test tube. Use a pipette to measure out the volumes. It is very important to accurately measure the amounts of Hydrogen Peroxide, Yeast
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Other cards in this set

Card 2

Front

enzyme

Back

Type of enzymes the same

Card 3

Front

reliability

Back

Preview of the front of card 3

Card 4

Front

measurement

Back

Preview of the front of card 4

Card 5

Front

same

Back

Preview of the front of card 5
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