Endo 3

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what is an immunoassay
measurment of the amount of given moleuclar compound/mass based on the interaction of hormone and antibody forming a complex.
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Name 3 different types of immunoassay
radioimmunoassay, fluoroimmunoassay, enzyme linked immunoassay
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How are immunoassays seperated- 2/3
using charcoal to trap the unbound hormone, cellulose seperates and forming a suspension to determine radioactivity
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What is an immunoradiometric assay
Type of immunometric assay that uses 2 different monoclonal antibodies that bind to different regions on 1 hormone to work out how much of each hormone is present
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What is ELISA
enzyme linked immunosorbent assay using an enzmye labelled detection antibody that causes the substrate to change colour
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4 advantages of immunoassay
V sensitive, specific and precise, also convenient/cost effective
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What is a bioassay
measures the magnitude/intensity of a biological effect e.g. cell growth
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What is a receptor assay
Using the whole tissue to fractionise a receptor and treat it with/like an immunoassay
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2 ads and 2 disads of receptor assay
High specificity for hormone and v sensitive but its labour intensive and needs animal/tissue processing
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Card 2

Front

Name 3 different types of immunoassay

Back

radioimmunoassay, fluoroimmunoassay, enzyme linked immunoassay

Card 3

Front

How are immunoassays seperated- 2/3

Back

Preview of the front of card 3

Card 4

Front

What is an immunoradiometric assay

Back

Preview of the front of card 4

Card 5

Front

What is ELISA

Back

Preview of the front of card 5
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