DNA Technology

Threw together some last minute questions. Helped me revise too :)

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  • Created by: dean
  • Created on: 21-06-12 16:01
What does RFLIP stand for?
Restriction Fragment Length Polymorphisms
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What is a primer used for during in vitro cloning.
Start sequence to initiate copying of DNA by DNA polymerase.
2 of 8
A sample of DNA is taken from each of two human males and cut with the same restriction enzyme. Why would the banding pattens of an electrophoresis machine of each sample be different when using the genetic fingerprinting technique?
Because the amount of introns vary from person to person.
3 of 8
Why would you not use in vivo gene cloning to analyse archaeological remains? ( give the most appropriate answer)
DNA may contain foreign DNA.
4 of 8
Plasmid X and Y contained the gene for antibiotic resistance to both Ampicillin and Tetracyclin. Plasmid X was inserted with a human gene and became a recombinant plasmid. When the plasmids X and Y were placed in agar gel containing Tetracyclin, only Y gr
The human gene disrupted the gene for resistance to tetracyclin on plasmid X.
5 of 8
What is the order of events that take place during fingerprinting.
extraction - digestion - seperation - hybridisation
6 of 8
A sequencer machine has a resolution of:
1 nucleotide.
7 of 8
The name of the enzyme that recombines two different gene regions of a recombinant plasmid is called:
DNA ligase
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Other cards in this set

Card 2

Front

What is a primer used for during in vitro cloning.

Back

Start sequence to initiate copying of DNA by DNA polymerase.

Card 3

Front

A sample of DNA is taken from each of two human males and cut with the same restriction enzyme. Why would the banding pattens of an electrophoresis machine of each sample be different when using the genetic fingerprinting technique?

Back

Preview of the front of card 3

Card 4

Front

Why would you not use in vivo gene cloning to analyse archaeological remains? ( give the most appropriate answer)

Back

Preview of the front of card 4

Card 5

Front

Plasmid X and Y contained the gene for antibiotic resistance to both Ampicillin and Tetracyclin. Plasmid X was inserted with a human gene and became a recombinant plasmid. When the plasmids X and Y were placed in agar gel containing Tetracyclin, only Y gr

Back

Preview of the front of card 5
View more cards

Comments

dean

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q.8 official answer wrong. 

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