Finding the order of DNA nucleotides or bases in a piece of DNA
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Methyl Interference Assay
Analytic method used to identify nucleotides that are important for DNA binding
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Primer
Strand of nucleinc acid that serves as a starting point for DNA synthesis
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Klenow Fragment
Protein fragment produced when DNA polymerase 1 is cut my the protease subtilisin enzyme
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Protease
Enzyme that conducts proteolysis
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Proteoloysis
breakdown of proteins into smaller polypeptides or amino acids. This generally occurs by the hydrolysis of the peptide bond, and is most commonly achieved by cellular enzymes called proteases
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Polyscrilamide gen electrophoresis
used to seperate DNA or protein molecules - high resolution allowing separation of single/ base pairs
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Genome Sequencing
Determination of the order of bases in the DNA of a genome
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Non-Protein coding sequences
All the sequences in DNA that do not contribute to making a protein - includes regulatory regions and pseudogenes
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Pseudogenes
ysfunctional relatives of genes that have lost their protein-coding ability or are otherwise no longer expressed in the cellG
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Genome map
Set of landmarks in the genome such as DNA sequences, regulatory sites and promotors so people can find specific genes they are after
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DNA sequencing
establishment of the order of nucleotide bases in a piece of DNA
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DNA sequencing methods
Maxam and Gilbert method, and Sanger Dieoxy method
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How identical are humans (%)
99.9%
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Other cards in this set
Card 2
Front
Methyl Interference Assay
Back
Analytic method used to identify nucleotides that are important for DNA binding
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